Oxidative stress
Background and utility
Lipid peroxidation, as a marker for oxidative stress, is known to be influenced by various pathological conditions, including iron overload and/or the presence of circulating toxic iron species NTBI and LPI. Malonaldehyde (MDA), one of the end products of lipid peroxidation, is the most commonly used marker for this process.
Technique(s)
As a measure of lipid peroxidation, MDA concentrations are measured in 150 µl plasma or serum in duplicate.1,2 The lower limit of detection of the method is 0.12 µM. The (reference) range of serum MDA as measured in an adult Dutch population is 0.16-0.64 µM.
- Conti M, Morand PC , Levillain P, Lemonnier A. Improved fluorometric determination of malonaldehyde. Clin Chem 1991, 37 (7): 1273-1275.
- van Eijk L, Heemskerk S, van der Pluijm R, van Wijk S, Peters W, van der Hoeven J, Kox M, Swinkels D, Pickkers P. The effect of iron loading and iron chelation on the innate immune response and subclinical organ injury during human endotoxinemia: a randomized trial. Haematologica. 2014, 99(3):579-587.
Responsible laboratory
Oxidative stress measurements are coordinated and performed by the Hepcidinanalysis.com initiative that is embedded in the Translational Metabolic Laboratory of the Department of Laboratory Medicine of the Radboudumc, Nijmegen, the Netherlands.
More information
Information about the assay and inquiries for projects can be requested via centerforirondisorders@radboudumc.nl
